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Bioss
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Merck KGaA
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Alomone Labs
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PhosphoSolutions
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CoCensys Inc
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Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of GABAA Rβ2 gene silencing results, individual duplex components or plasmids are also available upon request.
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CRISPR/Cas9 KO Plasmids consists of GABAA Rβ2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
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CRISPR/Cas9 KO Plasmids consists of GABAA Rβ2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
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CRISPR/Cas9 KO Plasmids consists of GABAA Rβ2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
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Buy from Supplier |
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Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of GABAA Rβ2 gene silencing results, individual duplex components or plasmids are also available upon request.
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Image Search Results
Journal: Molecular Neurodegeneration
Article Title: Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models
doi: 10.1186/s13024-021-00434-7
Figure Lengend Snippet: Information of the primary antibodies used in this study
Article Snippet:
Techniques:
Journal: bioRxiv
Article Title: Unravelling the molecular players at the cholinergic efferent synapse of the zebrafish lateral line
doi: 10.1101/2020.07.09.196246
Figure Lengend Snippet: Zebrafish α9 and α9α10 nAChRs are reversibly blocked by ɑ-Btx. Responses to 10 μM (α9) or 300 μM (α9α10) ACh either alone, in the presence of 100 nM α-Btx or after washing with control bath solution for 5 min, in oocytes expressing zebrafish α9 or α9α10 nAChRs are shown. Oocytes were pre-incubated with 100 nM α-Btx for 1 min prior to the addition of the agonist. α-Btx inhibited ACh responses by 93.70 ± 2.17% (n = 2) and 85.16 ± 6.86% (n= 2), respectively.
Article Snippet: All drugs were obtained from Sigma-Aldrich, except
Techniques: Expressing, Incubation
Journal: bioRxiv
Article Title: Unravelling the molecular players at the cholinergic efferent synapse of the zebrafish lateral line
doi: 10.1101/2020.07.09.196246
Figure Lengend Snippet: ACh-mediated inhibition of evoked Ca 2+ signals is blocked by α-Btx and apamin. A , Top , Representative temporal curves of mechanosensitive Ca 2+ responses of 4 HC, before (red) and after the application of 10 μM α-Btx (green). Bottom , Mechanosensitive Ca 2+ signals show no significant difference before and after 10 μM α-Btx treatment (Extra: Mdn ΔF/F0= 0.509 IQR: 0.252-1.134 Vs. α-Btx: Mdn ΔF/F0= 0.534 IQR: 0.331-1.325, n = 25, W= −45, p= 0.5449, MPRBC = 0.138). B , Top , Representative temporal curves of mechanosensitive Ca 2+ responses of 4 HC, after the application of 10 μM α-Btx (green) and after the co-application of 1 mM ACh and 10 μM α-Btx (blue). Bottom , When co-applied with 10 μM α-Btx, ACh-mediated inhibition is blocked (n = 25, W= −87, p= 0.2521, MPRBC = 0.268). C , Top , Representative temporal curves of mechanosensitive Ca 2+ responses of 4 hair cells, before (red) and after the application of 10 μM apamin (orange). Bottom , Mechanosensitive Ca 2+ signals show no significant difference before and after 10 μM apamin treatment (Extra: Mdn ΔF/F0= 0.599 IQR: 0.243-1.216 Vs. Apa: Mdn ΔF/F0= 0.567 IQR: 0.191-1.040, n = 41, W= −91, p= 0.5554, MPRBC = 0.106). D , Top , Representative temporal curves of mechanosensitive Ca 2+ responses of 4 HC, after the application of 10 μM apamin (orange) and after the co-application of 1 mM ACh and 10 μM apamin (blue). Bottom , ACh-mediated inhibition is blocked by 10 μM apamin (n = 60, W= −322, p= 0.2359, MPRBC = 0.1759). A Wilcoxon matched-pairs signed rank test was used in all cases. Extra: Extracellular imaging solution, ACh: Acetylcholine, α-Btx: α-bungarotoxin, Apa: Apamin. Horizontal scale bar in A , B , C and D : 1.5 sec, vertical scale bar in A , B , C and D : 25% ΔF/F0. Duration of the stimulus is indicated by gray lines below each trace.
Article Snippet: All drugs were obtained from Sigma-Aldrich, except
Techniques: Inhibition, Imaging